Journal: The Journal of Biological Chemistry

Article Title: Homeostatic Competition between Phasic and Tonic Inhibition *

doi: 10.1074/jbc.M113.491464

Figure Lengend Snippet: Ectopic expression of α6 subunit-induced δ subunit expression in pyramidal neurons. A and B, a control neuron transfected with mCherry (red) showed a very low level of δ immunofluorescence signal (green). C and D, two representative neurons transfected with the α6 subunit and mCherry showed increased an δ immunofluorescence signal on the soma and dendrites. Scale bars = 20 μm. E, quantified fluorescence intensity showing elevated δ expression in α6-transfected neurons (n = 24 in both groups). ***, p < 0.001, unpaired Student's t test. a.u., arbitrary units.

Article Snippet: Cultured neurons were transfected with mCherry alone or α6 plus mCherry, and the antibodies specific for the δ subunit (PhosphoSolutions) were used to label the neuronal surface expression in the non-permeabilized condition.

Techniques: Expressing, Control, Transfection, Immunofluorescence, Fluorescence

Journal: The Journal of Biological Chemistry

Article Title: Homeostatic Competition between Phasic and Tonic Inhibition *

doi: 10.1074/jbc.M113.491464

Figure Lengend Snippet: Overexpression of α5-GABAA receptors also decreased synaptic GABAergic transmission. A–C, control hippocampal neurons overexpressing mCherry showed no HA staining. D–F, hippocampal neurons overexpressing the HA-α5β3γ2 subunits showed a substantial HA staining signal throughout the soma and dendrites. G and H, representative traces showing reduced mIPSC events in hippocampal neurons overexpressing α5-GABAA receptors. Non-TF, non-transfected. I and J, quantitative data showing the reduction of mIPSC frequency but not the amplitude after overexpressing α5-GABAA receptors (n = 10–11). ***, p < 0.001, Student's t test. Scale bars = 20 μm for A, B, D, and E and 5 μm for C and F.

Article Snippet: Cultured neurons were transfected with mCherry alone or α6 plus mCherry, and the antibodies specific for the δ subunit (PhosphoSolutions) were used to label the neuronal surface expression in the non-permeabilized condition.

Techniques: Over Expression, Transmission Assay, Control, Staining, Transfection

Journal: Ecotoxicology and environmental safety

Article Title: Additive cardiotoxicity of a bisphenol mixture in zebrafish embryos: The involvement of calcium channel and pump.

doi: 10.1016/j.ecoenv.2023.115225

Figure Lengend Snippet: Fig. 4. Effects of BPs on cardiomyocyte development. Representative confocal microscopy image of TG(myl7:NLS-mCherry) ZFEs at 48 hpf exposed to 0.05% DMSO (A) or Mix 2 (BPA 8.7 µM + BPF 18 µM + BPAF 1.3 µM + BPB 4.2 µM), which induced approximately 40% heart rate reduction (B). BP exposure did not affect the cardiomyocyte count (C), based on the t test (n = 10). BP exposure, which induced approximately 30% heart rate reduction, did not affect mRNA expression of genes related to cardiomyocyte development: hand2 (D) and nkx20.5 (E). Only BPF and BPAF significantly downregulated bmp4 mRNA expression (F), based on the one- way ANOVA test followed by Dunnett’s post hoc analysis (n = 3). SC: DMSO 0.05%, BPA: 25 µM, BPF: 60 µM, BPAF: 4 µM, BPB: 12 µM, Mix 1: BPA 6.6 µM + BPF 13.6 µM + BPAF 1 µM + BPB 3.2 µM. ns, P ≥0.03; *, P ≤0.03; **, P ≤0.002; *** , P ≤0.0002; *** *, P ≤0.0001. The data are presented as means ± standard deviations.

Article Snippet: Embryos were stained with mCherry Mouse Polyclonal Antibody (Cusabio, Houston, TX, USA), Goat Anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, and Alexa Fluor Plus 594 (Invitrogen) according to a previously described protocol (Hammond-Weinberger and ZeRuth, 2020).

Techniques: Confocal Microscopy, Expressing